RNA

Part:BBa_K4604007

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)


K12 riboswitch for AdoCbl

A riboswitch is a RNA sequence capable of binding to a certain molecule. In the case of BioBrick BBa_K4604000 this molecule is Adenosylcobalamin (AdoCbl). Upon binding to AdoCbl the structure of the riboswitch changes which in turn blocks the availability of a ribosome binding site (RBS). This RBS is part of the riboswitch and needed to start the translation of the gene downstream of it. In the presence of AdoCbl the gene after the riboswitch is negatively regulated on should not be expressed. BioBrick BBa_K4604007 is used in Biobricks...

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 162
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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